华进联
一、个人基本信息
华进联，博士，西北农林科技大学动物医学院三级教授，西北农林科技大学动物生物技术研究方向博士生指导教师。陕西省干细胞工程技术研究中心主任，陕西省重点科技创新团队负责人，教育部新世纪优秀人才，陕西省中青年科技创新领军人才。2022年度学校优秀研究生导师团队负责人。
二、教育经历
1990.9-1995.7, 西北农业大学动物医学系，获农学学士学位; 1998.9-2001.7, 西北农业大学动物科技学院获临床兽医学硕士学位; 2001.9-2005.7, 西北农业大学动物科技学院获临床兽医学博士学位。
三、研究工作经历
1995.7-2000.12，西北农林科技大学秦巴研究中心实习研究员；2001.1-2005.12，西北农林科技大学动物科技学院助理研究员；2006.1-2010.12，西北农林科技大学动物医学院副研究员；2005.8-2006.8，澳大利亚新南威尔士大学医学院糖尿病移植中心博士后（访问学者）；2011.1至今，西北农林科技大学动物医学院基础兽医系研究员。
四、学术兼职
陕西省干细胞学会副理事长、陕西省抗癌协会肿瘤标志委员会常委、中国动物学会生殖生物学分会理事、中国动物学会细胞与分子学分会理事、中国生理学会生殖生物学分会理事、中国生物化学与分子生物学会农业生物化学与分子生物学会理事。系《Cell Proliferation》、《Journal of Stem Cells and Clinical Practice》《World Journal of Stem Cells ( WJSC)》《Journal of Integrative Agriculture》《中国农业科学》《生命的化学》等学术期刊编委。
五、科学研究
研究方向为干细胞与生殖细胞发育调控、干细胞抗病育种及重大疾病防控。在动物和人类胚胎干细胞、雄性生殖干细胞和iPS细胞发育调控及干细胞介导培育抗布氏杆菌山羊，抗蓝耳病猪育种材料和细胞模型；干细胞治疗糖尿病、肝损伤等代谢性疾病和繁殖障碍性疾病等重大疾病防控研究研究上取得了有特色的研究进展，获得了一批干细胞新制剂。
主持承担国家和省部级以上科研项目近20项。获陕西省科技一等奖2项，二等奖4项，申请国家发明专利15项，授权专利8项。发表论文200余篇。主编或受邀参与出版著作8部。
发表的主要相关论文：
1. Juqing Zhang,Yaqi Zhou,Wei Yue, Zhenshuo Zhu, Xiaolong Wu, Shuai Yu, Qiaoyan Shen, Qin Pan, Wenjing Xu, Rui Zhang, Xiaojie Wu, Xinmei Li, Yayu Li, Yunxiang Li, Yu Wang, Sha Peng, Shiqiang Zhang, Anmin Lei, Xinbao Ding, Fan Yang, Xingqi Chen,Na Li, Mingzhi Liao, Wei Wang, and Jinlian Hua. Super-enhancers conserved within placental mammals maintain stem cell pluripotency. 2022, September 26, 119 (40):e2204716119. https://doi.org/10.1073/pnas.2204716119.
2. Ren Y, Aierken A, Zhao L, Lin Z, Jiang J, Li B, Wang J, Hua J, Tu Q. hUC-MSCs lyophilized powder loaded polysaccharide ulvan driven functional hydrogel for chronic diabetic wound healing. Carbohydr Polym. 2022 Jul 15;288:119404. doi: 10.1016/j.carbpol.2022.119404. Epub 2022 Mar 28. PMID: 35450656.
3. Ju-Qing Zhang, Jian-Xiong Guo, Xiao-Jie Wu, Yu Wang, Wei Yue, Zhuo-Hui Li, Wei Wang, Xin-Fang Wei, Xiao-Long Wu, Qiao-Yan Shen, An-Min Lei , Hong-Jiang Wei, Jin-Lian Hua. Optimization of sgRNA expression strategy to generate multiplex gene-edited pigs. Zoological Research, 2022,43(6): 1005-1008.
4. Wenjing Xu, Yumei Yang, Yunxiang Li, Donghui Yang, Shicheng Wan, Na Li, Jinlian Hua. Spliced X-box binding protein 1 (XBP1s) protects spermatogonial stem cells (SSCs) from lipopolysaccharide (LPS)-induced damage by regulating the testicular microenvironment. Theriogenology,1 October 2022, 191:132-140.
5. Xin-Chun Yang, Xiao-Long Wu, Wen-Hao Li, Xiao-Jie Wu, Qiao-Yan Shen, Yun-Xiang Li, Sha Peng, Jin-Lian Hua. OCT6 inhibits differentiation of porcine-induced pluripotent stem cells through MAPK and PI3K signaling regulation. Zoological Research, 2022, 43(6): 911-922. doi: 10.24272/j.issn.2095-8137.2022.220
6. Zhang Y, de Lima CB, Zhou Z, Shen Q, Zhu Z, Hua J. Peptide-coating 2D and small chemical molecules prolong the passage of porcine spermatogonia stem cells. Reprod Domest Anim. 2022 Feb;57(2):200-209. doi: 10.1111/rda.14042. Epub 2021 Dec 5. PMID: 34748668.
7. Aierken A, Li B, Liu P, Cheng X, Kou Z, Tan N, Zhang M, Yu S, Shen Q, Du X, Enkhbaatar BB, Zhang J, Zhang R, Wu X, Wang R, He X, Li N, Peng S, Jia W, Wang C, Hua J. Melatonin treatment improves human umbilical cord mesenchymal stem cell therapy in a mouse model of type II diabetes mellitus via the PI3K/AKT signaling pathway. Stem Cell Res Ther. 2022 Apr 12;13(1):164. doi: 10.1186/s13287-022-02832-0. PMID: 35414044; PMCID: PMC9006413.
8. Tian H, Niu H, Luo J, Yao W, Chen X, Wu J, Geng Y, Gao W, Lei A, Gao Z, Tian X, Zhao X, Shi H, Li C, Hua J. Knockout of Stearoyl-CoA Desaturase 1 Decreased Milk Fat and Unsaturated Fatty Acid Contents of the Goat Model Generated by CRISPR/Cas9. J Agric Food Chem. 2022 Apr 6;70(13):4030-4043. doi: 10.1021/acs.jafc.2c00642. Epub 2022 Mar 28. PMID: 35343224.
9. Yu XW, Li TT, Du XM, Shen QY, Zhang MF, Wei YD, Yang DH, Xu WJ, Chen WB, Bai CL, Li XL, Li GP, Li N, Peng S, Liao MZ, Hua JL. Single-cell RNA sequencing reveals atlas of dairy goat testis cells. Zool Res. 2021 Jul 18;42(4):401-405. doi: 10.24272/j.issn.2095-8137.2020.373. PMID: 34047080; PMCID: PMC8317185.
10. Zhu Z, Pan Q, Zhao W, Wu X, Yu S, Shen Q, Zhang J, Yue W, Peng S, Li N, Zhang S, Lei A, Hua J. BCL2 enhances survival of porcine pluripotent stem cells through promoting FGFR2. Cell Prolif. 2021 Jan;54(1):e12932. doi: 10.1111/cpr.12932. Epub 2020 Oct 26. PMID: 33107129; PMCID: PMC7791183.
11. Yang D, Wei Y, Lu Q, Qin D, Zhang M, Du X, Xu W, Yu X, He C, Li N, Peng S, Li G, Hua J. Melatonin alleviates LPS-induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551. doi: 10.1002/jcp.30088. Epub 2020 Sep 29. PMID: 32996162.
12. Zhang M, Li N, Liu W, Du X, Wei Y, Yang D, Zhou Z, Ma F, Peng S, Zhang S, He X, Bai C, Li G, Hua J. Eif2s3y Promotes the Proliferation of Spermatogonial Stem Cells by Activating ERK Signaling. Stem Cells Int. 2021 Jan 29;2021:6668658. doi: 10.1155/2021/6668658. PMID: 33603791; PMCID: PMC7869416.
13. Wei Y, Yang D, Du X, Yu X, Zhang M, Tang F, Ma F, Li N, Bai C, Li G, Hua J. Interaction between DMRT1 and PLZF protein regulates self-renewal and proliferation in male germline stem cells. Mol Cell Biochem. 2021 Feb;476(2):1123-1134. doi: 10.1007/s11010-020-03977-3. Epub 2020 Nov 16. PMID: 33200378.
14. Zhu Z, Wu X, Li Q, Zhang J, Yu S, Shen Q, Zhou Z, Pan Q, Yue W, Qin D, Zhang Y, Zhao W, Zhang R, Peng S, Li N, Zhang S, Lei A, Miao YL, Liu Z, Chen X, Wang H, Liao M, Hua J. Histone demethylase complexes KDM3A and KDM3B cooperate with OCT4/SOX2 to define a pluripotency gene regulatory network. FASEB J. 2021 Jun;35(6):e21664. doi: 10.1096/fj.202100230R.
15. Wei YD, Du XM, Yang DH, Ma FL, Yu XW, Zhang MF, Li N, Peng S, Liao MZ, Li GP, Bai CL, Liu WS, Hua JL. Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells. Zool Res. 2021 Jan 18;42(1):14-27. doi: 10.24272/j.issn.2095-8137.2020.186. PMID: 33420764; PMCID: PMC7840460.
16. Li N, Du Z, Li Y, Xu W, Yang Y, Peng H, Song T, Qin Q, Lei H, Hua J. p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation. Stem Cells Int. 2021 Dec 24;2021:4968649. doi: 10.1155/2021/4968649. PMID: 34976070; PMCID: PMC8720024.
17. Yu S, Zhang R, Shen Q, Zhu Z, Zhang J, Wu X, Zhao W, Li N, Yang F, Wei H, Hua J. ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2. Front Cell Dev Biol. 2021 Sep 20;9:712224. doi: 10.3389/fcell.2021.712224. PMID: 34616727; PMCID: PMC8488167.
18. Li B, Cheng X, Aierken A, Du J, He W, Zhang M, Tan N, Kou Z, Peng S, Jia W, Tang H, Hua J. Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway. Front Cell Dev Biol. 2021 Oct 15;9:722365. doi: 10.3389/fcell.2021.722365.
19. Wu XL, Zhu ZS, Xiao X, Zhou Z, Yu S, Shen QY, Zhang JQ, Yue W, Zhang R, He X, Peng S, Zhang SQ, Li N, Liao MZ, Hua JL. LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells. Zool Res. 2021 May 18;42(3):377-388. doi: 10.24272/j.issn.2095-8137.2020.375. PMID: 33998185; PMCID: PMC8175949.
20. Li T, Li Q, Li H, Xiao X, Ahmad Warraich D, Zhang N, Chen Z, Hou J, Liu T, Weng X, Liu Z, Hua J, Liao M. Pig-specific RNA editing during early embryo development revealed by genome-wide comparisons. FEBS Open Bio. 2020 Jul;10(7):1389-1402. doi: 10.1002/2211-5463.12900. Epub 2020 Jun 25. PMID: 32433824; PMCID: PMC7327910.
21. Liu W, Li N, Zhang M, Liu Y, Sun J, Zhang S, Peng S, Hua J. Eif2s3y regulates the proliferation of spermatogonial stem cells via Wnt6/<beta>-catenin signaling pathway. Biochim Biophys Acta Mol Cell Res. 2020 Oct;1867(10):118790. doi: 10.1016/j.bbamcr.2020.118790. Epub 2020 Jul 1. PMID: 32621839.
22. Sun C, Zhang N, Yu P, Wu X, Li Q, Li T, Li H, Xiao X, Shalmani A, Li L, Che D, Wang X, Zhang P, Chen Z, Liu T, Zhao J, Hua J, Liao M. Enhancer recognition and prediction during spermatogenesis based on deep convolutional neural networks. Mol Omics. 2020 Oct 1;16(5):455-464. doi: 10.1039/d0mo00031k. Epub 2020 Jun 22. PMID: 32568326.
23. Zhu H, Zheng L, Wang L, Tang F, Arisha AH, Zhou H, Hua J. p53 inhibits the proliferation of male germline stem cells from dairy goat cultured on poly-L- lysine. Reprod Domest Anim. 2020 Mar;55(3):405-417. doi: 10.1111/rda.13645. Epub 2020 Feb 17. PMID: 31985843.
24. Shen Q, Xiao X, Aierken A, Yue W, Wu X, Liao M, Hua J. The ACE2 expression in Sertoli cells and germ cells may cause male reproductive disorder after SARS- CoV-2 infection. J Cell Mol Med. 2020 Aug;24(16):9472-9477. doi: 10.1111/jcmm.15541. Epub 2020 Jun 28. PMID: 32594644; PMCID: PMC7361928.
25. Shen QY, Yu S, Zhang Y, Zhou Z, Zhu ZS, Pan Q, Lv S, Niu HM, Li N, Peng S, Liao MZ, Wang HY, Lei AM, Miao YL, Liu ZH, Hua JL. Characterization of porcine extraembryonic endoderm cells. Cell Prolif. 2019 May;52(3):e12591. doi: 10.1111/cpr.12591. Epub 2019 Mar 21. PMID: 30896067; PMCID: PMC6536407.
26. Ma F, Du X, Wei Y, Zhou Z, Clotaire DZJ, Li N, Peng S, Li G, Hua J. LIN28A activates the transcription of NANOG in dairy goat male germline stem cells. J Cell Physiol. 2019 Jun;234(6):8113-8121. doi: 10.1002/jcp.27593. Epub 2018 Oct 14. PMID: 30317605.
27. Zhang J, Zhu Z, Yue W, Li J, Chen Q, Yan Y, Lei A, Hua J. Establishment of CRISPR/Cas9-Mediated Knock-in System for Porcine Cells with High Efficiency. Appl Biochem Biotechnol. 2019 Sep;189(1):26-36. doi: 10.1007/s12010-019-02984-5. Epub 2019 Mar 12. PMID: 30859452.
28. Clotaire DZJ, Wei Y, Yu X, Ousman T, Hua J. Functions of promyelocytic leukaemia zinc finger (Plzf) in male germline stem cell development and differentiation. Reprod Fertil Dev. 2019 Apr 23. doi: 10.1071/RD18252. Epub ahead of print. PMID: 31009592.
29. Yan Y, Fang J, Wen X, Teng X, Li B, Zhou Z, Peng S, Arisha AH, Liu W, Hua J. Therapeutic applications of adipose-derived mesenchymal stem cells on acute liver injury in canines. Res Vet Sci. 2019 Oct;126:233-239.
30. Wang X, Wu X, Zhu Z, Li H, Li T, Li Q, Zhang P, Li L, Che D, Xiao X, Liu T, Hua J, Liao M. Landscape of RNA editing reveals new insights into the dynamic gene regulation of spermatogenesis. Cell Cycle. 2019 Dec;18(23):3351-3364.
31. Li N, Ma W, Shen Q, Zhang M, Du Z, Wu C, Niu B, Liu W, Hua J. Reconstitution of male germline cell specification from mouse embryonic stem cells using defined factors in vitro. Cell Death Differ. 2019 Jan 25. doi: 10.1038/s41418-019-0280-2. [Epub ahead of print]
32. Li B, He X, Zhuang M, Niu B, Wu C, Mu H, Tang F, Cui Y, Liu W, Zhao B, Peng S, Li G, Hua J. Melatonin Ameliorates Busulfan-Induced Spermatogonial Stem Cell Oxidative Apoptosis in Mouse Testes. Antioxid Redox Signal. 2018 Feb 10;28(5):385-400.（ESI高被引论文）
33. Clotaire DZJ, Du X, Wei Y, Yang D, Hua J.miR-19b-3p integrates Jak-Stat signaling pathway through Plzf to regulate self-renewal in dairy goat male germline stem cells. Int J Biochem Cell Biol. 2018 Dec;105:104-114.
34. Fang J, Yan Y, Teng X, Wen X, Li N, Peng S, Liu W, Donadeu FX, Zhao S, Hua J. Melatonin prevents senescence of canine adipose-derived mesenchymal stem cells through activating NRF2 and inhibiting ER stress. Aging (Albany NY). 2018 Oct 25;10(10):2954-2972.
35. Du Z, Xu S, Hu S, Yang H, Zhou Z, Sidhu K, Miao Y, Liu Z, Shen W, Reiter RJ, Hua J, Peng S. Melatonin attenuates detrimental effects of diabetes on the niche of mouse spermatogonial stem cells by maintaining Leydig cells. Cell Death Dis. 2018 Sep 20;9(10):968.
36. Lei Q, Pan Q, Li N, Zhou Z, Zhang J, He X, Peng S, Li G, Sidhu K, Chen S, Hua J. H19 regulates the proliferation of bovine male germline stem cells via IGF-1 signaling pathway. J Cell Physiol. 2018 Jan;234(1):915-926.
37. Daguia Zambe JC, Zhai Y, Zhou Z, Du X, Wei Y, Ma F, Hua J. miR-19b-3p induces cell proliferation and reduces heterochromatin-mediated senescence through PLZF in goat male germline stem cells. J Cell Physiol. 2018 Jun;233(6):4652-4665.
38. Wei Y, Cai S, Ma F, Zhang Y, Zhou Z, Xu S, Zhang M, Peng S, Hua J. Double sex and mab-3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells. J Cell Physiol. 2018 Mar;233(3):2537-2548.
39. Li N, Hua J. Interactions between mesenchymal stem cells and the immune system.Cell Mol Life Sci. 2017 Jul;74(13):2345-2360.
40. Niu Z, Mu H, Zhu H, Wu J, Hua J.p38 MAPK pathway is essential for self-renewal of mouse male germline stem cells (mGSCs).Cell Prolif. 2017 Feb;50(1). doi: 10.1111/cpr.12314. Epub 2016 Nov 21.
41. Niu ZW, Zheng LM, Wu SY, Mu HL, Ma FL, Song WC, Zhu HJ, Wu J, He X, Hua JL. Ras/ERK1/2 pathway regulates the self-renewal of dairy goat spermatogonia stem cells. Reproduction, 2015, 149: 445-452 DOI: 10.1530/rep-14-0506.
42. Song, W.C., H.L. Mu, J. Wu, M.Z. Liao, H.J. Zhu, L.M. Zheng, X. He, B.W. Niu, Y.X. Zhai, C.L. Bai, A.M. Lei, G.P. Li, J.L. Hua. miR-544 Regulates Dairy Goat Male Germline Stem Cell Self-Renewal via Targeting PLZF. Journal of Cellular Biochemistry, 2015:116, 2155-2165 DOI: 10.1002/jcb.25172.
43. Mu H, Li N, Wu J, Zheng L, Zhai Y, Li B, Song W, Wang J, Zhu H, Li G, Hua J. PLZF-Induced Upregulation of CXCR4 Promotes Dairy Goat Male Germline Stem Cell Proliferation by Targeting Mir146a. J Cell Biochem, 2015, Sep 14. doi: 10.1002/jcb.25371. [Epub ahead of print]
44. Niu B, Wu J, Hua J. miR-204 regulated the proliferation of dairy goat spermatogonial stem cells via targeting to Sirt1. Rejuvenation Res. 2015, Jul 27. [Epub ahead of print]
45. Han W, He X, Zhang M, Hu S, Sun F, Ren L, Hua J, Peng S. Establishment of a porcine pancreatic stem cell line using T-REx(™) system-inducible Wnt3a expression.Cell Prolif. 2015 Jun, 48(3):301-10.
46. He X, Han W, Hu SX, Zhang MZ, Hua JL, Peng S. Canonical Wnt signaling pathway contributes to the proliferation and survival in porcine pancreatic stem cells (PSCs).Cell Tissue Res. 2015, Nov;362(2):379-88. doi: 10.1007/s00441-015-2220-x. Epub 2015 Jun 18.
47. Zhu, H.J., J. Ma, R. Du, L.M. Zheng, J. Wu, W.C. Song, Z.W. Niu, X. He, E.Q. Du, S.T. Zhao, J.L. Hua. Characterization of Immortalized Dairy Goat male Germline Stem Cells (mGSCs). Journal of Cellular Biochemistry. 2014, 115:1549-1560 DOI: 10.1002/jcb.24812.
48. Wu, J., M.Z. Liao, H.J. Zhu, K. Kang, H.L. Mu, W.C. Song, Z.W. Niu, X. He, C.L. Bai, G.P. Li, X.P. Li, J.L. Hua. CD49f-Positive Testicular Cells in Saanen Dairy Goat Were Identified as Spermatogonia-Like Cells by miRNA Profiling Analysis. Journal of Cellular Biochemistry. 2014, 115:1712-1723 DOI: 10.1002/jcb.24835.
49. Yu M, Mu HL, Niu ZW, Chu ZL, Zhu HJ, Hua JL. miR-34c Enhances Mouse Spermatogonial Stem Cells Differentiation by Targeting Nanos2. Journal of Cellular Biochemistry. 2014, 115, 232-242 DOI: 10.1002/jcb.24655.
50. Tang F, Yao X, Zhu H, Mu H, Niu Z, Yu M, Yang C, Peng S, Li G, Hua J. Expression pattern of Ngn3 in dairy goat testis and its function in promoting meiosis by upregulating Stra8. Cell Prolif, 2014, 47(1):38-47.
51. Li M, Yu M, Liu C, Zhu H, He X, Peng S, Hua J. miR-34c works downstream of p53 leading to dairy goat male germline stem-cell (mGSCs) apoptosis. Cell Prolif, 2013, 46(2):223-31.
52. Wu J, Song W, Zhu H, Niu Z, Mu H, Lei A, Yang C, Peng S, Li X, Li G, Hua J. (2013) Enrichment and characterization of Thy1-positive male germline stem cells (mGSCs) from dairy goat (Capra hircus) testis using magnetic microbeads. Theriogenology, 2013, 80(9):1052-1060.
53. Hua J, Zhu H, Pan Si, Liu C, Sun J, Ma X, Dong W, Liu W, Li W. Pluripotent male germline stem cells from goat fetal testis and their survival in mouse testis. Cellular Reprogramming, 2011, 13(2): 133-144.
54. Hua J, Yu H, Liu S, Dou Z, Sun Y, Jing X, Yang C, Lei A, Wang H, Gao Z. Derivation, characterization of human embryonic germ cells: serum-free culture and potentiality of germ cell differentiation. Biomed Reprod Online, 2009, 19: 238-249.
55. Hua J, Pan S, Yang C, Dong W, Dou Z, Sidhu KS. Derivation of male germ cell-like lineage from human bone marrow stem cells. Biomed Reprod Online, 2009, 19:99-105.
56. Hua J, Yu H, Dong W, Yang C, Gao Z, Lei A, Sun Y, Pan S, Wu Y, Dou Z. Characterization of mesenchymal stem cells (MSCs) from human fetal lung:Potential differentiation of germ cells. Tissue and Cell, 2009, 41:448-455.
57. Hua J, Sidhu KS. Recent advances in the derivation of germ cells from the embryonic stem cells. Stem Cells and Development, 2008, 17:399-411.
六、联系方式
e-mail： jinlianhua@nwsuaf.edu.cn, jlhua2003@126.com