王勇胜

作者: 来源: 发布日期:2019-05-07 浏览次数:

  一、个人基本信息

  王勇胜,男,1977年10月出生,博士,四级教授,博士生导师。

  二、教育及研究经历

  教育经历(从大学本科开始,按时间倒排序):

  2004-2009 年 西北农林科技大学临床兽医专业  获博士学位;

  2001-2004年 西北农林科技大学临床兽医专业  获得硕士学位;

  1997-2001年 西北农林科技大学动物医学院    获得学士学位;

  工作经历(科研与学术工作经历,按时间倒排序):

  2019 -至今,任职于西北农林科技大学动物医学学院,教授;2013-2018年,任职于西北农林科技大学动物医学学院,副教授;

  2012-2013年,任职于西北农林科技大学动物医学学院,讲师;

  2009-2012年,西北农林科技大学发育生物学博士后站,博士后;

  三、研究方向及开设课程

  研究方向:

  主要开展乳动物早期胚胎发育机理、胚胎重编程及表观遗传学、抗病转基因牛新品种的培育、体外受精和体细胞克隆等方面的研究。

  开设课程:

  主讲本科生兽医学专业《动物组织与胚胎学》和《动物组织与胚胎学实验》;动科和生科专业《动物解剖与组织胚胎学》和《动物解剖与组织胚胎学实验》;承担博士生《细胞与胚胎工程专题》和《动物生物学专题》等课程。

  四、代表性学术成果

  1. Yuanpeng Gao#, Haibo Wu#, Yongsheng Wang#, Xin Liu, Linlin Chen, Qian Li, Chenchen Cui, Xu Liu, Jingcheng Zhang, Yong Zhang*.Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off target effects. Genome Biology. 2017, 18:13.

  2. Jingcheng Zhang, Pengxiang Qu, Chuan Zhou, Xin Liu, Xiaonan Ma, Mengyun Wang, Yongsheng Wang*, Jianmin Su, Jun Liu, Yong Zhang*. MicroRNA-125b is a key epigenetic regulatory factor that promotes nuclear transfer reprogramming. The Journal of Biological Chemistry. 2017, 292, 15916-15926.

  3. Mengyun Wang, Yang Gao, Pengxiang Qu, Suzhu Qing, Fang Qiao, Yong Zhang,Jesse Mager,Yongsheng Wang*. Sperm-borne miR-449b influences cleavage, epigenetic reprogramming and apoptosis of SCNT embryos in bovine. Scientific Reports. 2017,7:1-12.

  4. Pengxiang Qu, Suzhu Qing, Ruiqi Liu, Hongyu Qin, Weiwei Wang, Fang Qiao,Hui Ge, Jun Liu, Yong Zhang, Wei Cui, Yongsheng Wang*. Effects of embryo-derived exosomes on the development of bovine cloned embryos. Plos One. 2017, 12(3)

  5. Yong Sheng Wang, Xiaoning He, Yue Du, Jianmin Su, Mingqing Gao, Yefei Ma, Song Hua, Fusheng Quan, Jun Liu,Yong Zhang. Transgenic cattle produced by nuclear transfer of fetal fibroblasts carrying Ipr1 gene at a specific locus. Theriogenology. 2015, 84: 608-616.

  6. Du Yue, Wang Xiangguo, Wang Bo, Wang Yongsheng* , Zhang Yong*. Deep sequencing analysis of microRNAs in bovine sperm. Molecular Reproduction and Development. 2014. 81(11): 1042-52.

  7. Qu Pengxiang,Li Yanyan,Deng Tengfei,Dan Jia,Qing Suzhu,Su Jianmin,Zhang Yong,Wang Yongsheng* . Effects of Insulin-like Growth Factor-1 on Development of Somatic Cell ClonedBovine Embryos. Cell Reprogramming. 2016,18(3):162-70.

  8. Xiaonan Ma, Ying Zhang, Fang Qiao, Mengyun Wang, Zhenzi Zuo, Yong Zhang, Yongsheng Wang*. Comparison of RNA extraction and microRNA detection protocols for a small amount of germinal vesicle oocytes in bovine. Animal Reproduction Science. 2018,195:112-120.

  9. Fang Qiao, Hui Ge, Xiaonan Ma, Ying Zhang, Zhenzi Zuo, Mengyun Wang, Yong Zhang, Yongsheng Wang*. Bovine uterus-derived exosomes improve developmental competence of somatic cell nuclear transfer embryos.  Theriogenology ,2018, (114) 199-205.

  10. Pan Shaohui, Chen W, Liu X, Xiao J, Wang Y, Liu J, Du Y, Wang Yongsheng*,, Zhang Y*. Application of a novel population of multipotent stem cells derived from skin fibroblasts as donor cells in bovine SCNT. PloS One. 2015 20;10(1):e0114423.

  11. Haibo Wu, Wang Yongsheng, Yan Zhang, Mingqi Yang, Jiaxing Lv, Jun Liu, and Yong Zhang. TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis. PNAS . 2015. 112(3): E1530-1539.

  12. Xu Liu, Wang Yongsheng, Wenjiang Guo, Bohao Chang, Jun Liu, Zekun Guo, Yong Zhang. Zinc-finger nickase-mediated insertion of the lysostaphin gene into the beta-casein locus in cloned cows. Nature Communications. 2013(4):1-11.

  13. Liu Xu, Wang Yongsheng, Tian Y C, Yu Y, Gao M Q, Hu G D, Su F, Pan S H, Luo Y, Guo Z K, Quan F S, and Zhang Y. Generation of mastitis resistance in cows by targeting human lysozyme gene to beta-casein locus using zinc-finger nucleases. Proceedings of the Royal Society B-Biological Sciences , 2014. 281:1780-1786.

  14. Yuan Yu, Wang Yongsheng#, Qi Tong, Xu Liu, Feng Su, Fusheng Quan, Zekun Guo, Yong Zhang. A Site-Specific Recombinase-Based Method to Produce Antibiotic Selectable Marker Free Transgenic Cattle. PLoS ONE . 2016, 8(5): e62457. doi:10.1371/journal.pone.0062457.

  15. Jianmin Su, Wang Yongsheng#, Lei Zhang, Bo Wang, Jun Liu, Yan Luo,  Zekun Guo, Fusheng Quan, Yong Zhang.Oocyte-Secreted Factors in Oocyte Maturation Media Enhance Subsequent Development of Bovine Cloned Embryos. Molecular Reproduction & Development . 2014. 81 (4): 341-349.

  16. Ying Zhang , Pengxiang Qu , Xiaonan Ma, Yong Zhang, Yongsheng Wang*, Wei cui. Tauroursodeoxycholic acid (TUDCA) alleviates endoplasmic reticulum stress of nuclear donor cells under serum starvation. PLOS ONE, 2018, 13(5):e0196785.

  17. Yongsheng Wang, Yijun Zhang, Tingchao Mao, Beifeng Yan, Ruizhi Deng, Biao Wei, Yong Zhang, Jun Liu Treatment donor cells with UNC0638 modify the abnormal histone H3K9 dimethylation and gene expression in cloned goat embryos. Small Ruminant Research . 2017 (156), 27-32.

  五、科研项目

      (1) 胚胎发育相关精子特异miRNAs鉴定及功能研究(2015.1-2018.12),国家自然科学基金面上项目(No. 31201940),87万,项目主持人。

  (2) Ipr1基因介导巨噬细胞凋亡的作用机制研究 (2013.1-2015.12),国家自然科学基金(No. 31472094),21 万,项目主持人。

  (3)抗结核病,布鲁斯杆菌病转基因牛新品种培育(2012.1-2014.12),转基因重大专项(2008ZX08007-004),113万,子项目主持人。

  (4)抗结核病转基因牛新品种培育(2016.1-2020.12),国家转基因重大专项(2016ZX08007003),   629.31万,子项主持人。(5) Xist干扰对牛体细胞克隆胚发育的影响 (2013.1-2015.12),教育部高校基本科研业务费,10万,项目主持人。 (6)牛皮肤Muse细胞的分离、鉴定和核移植效率研究 (2012.12-2014.12),西北农林科技大学博士科研启动基金,5万,项目主持人。 (7)家畜卵母细胞冷冻、胚胎表观修饰和体细胞克隆技术研究(2008AA101006),863重点课题,987万, 2011.1-2015.12,主要参加人。

  六、荣誉及奖项

  2013年陕西省科技进步一等奖“牛羊良种繁育关键技术研究与应用”,排名第二。

  2015年陕西省科技进步一等奖“牛羊基因定点精确编辑技术研究与应用”,排名第四。

  2016年国家技术发明二等奖“良种牛羊高效克隆技术”一项,排名第五。

  七、联系方式

  通讯地址:陕西省杨凌农业高新技术示范区 西北农林科技大学南校区科研主楼106信箱,2401室,  邮编 712100

  联系电话:029-87080092;  Email:manyno@126.com;wangyongsheng01@nwsuaf.edu.cn