许信刚
一、个人基本信息
许信刚，男，陕西武功人，博士，教授，博士生导师。
国家绒毛用羊细菌病防控团队成员、陕西省科技特派员，陕西省“三区”人才、教育部学位论文通讯评议专家，中国微生物学会兽医微生物学专业委员会青年委员、陕西省畜牧兽医学会会员。《动物医学进展》杂志编委。Vaccine、Veterinary Microbiology、Journal of Virological Methods、Acta Virologica、Virology Journal、Journal of General Virology、Virus Research、Journal of Virology等SCI杂志的特邀审稿人，《畜牧兽医学报》、《中国农业科学》、《西北农林科技大学学报（自然科学版）》、《中国兽医科学》、《西北农业学报》杂志审稿专家。
二、教育及研究经历
1997.7毕业于西北农林科技大学动物医学院兽医专业，获学士学位；2000.7毕业于西北农林科技大学预防兽医学专业，获硕士学位；2005.7毕业于西北农林科技大学临床兽医学专业，获博士学位。2006.4-2007.4月在台湾屏东科技大学兽医学院做博士后，主要进行动物基因工程疫苗的研究； 2013.4-2014.4月通过国家留学基金委资助在美国佐治亚大学兽医学院做访问学者，主要从事动物病毒与宿主相互作用的研究。
三、研究方向及开设课程
（一）研究方向
1.分子病原学与免疫学，重点开展：
（1）猪重要病毒性腹泻致病机理研究（猪流行性腹泻病毒）；
（2）动物重要疫病防控新型制剂研发（基因工程疫苗）；
（3）牛羊重要病原致病机理研究；
2.动物传染病学，重点开展：
（1）牛羊疫病快速诊断与防控新技术研究；
（2）兽医生物安全与综合防控。
（二）开设课程
1.本科生：《兽医微生物学》、《兽医微生物学实验》、《水产动物微生物学》、《动物微生物学实验技术》等课程；
2.硕士研究生：《兽医病原学专题》；《羊病防治与高效繁殖技术》；《反刍动物疫病学进展》
3.博士研究生：《兽医分子微生物学》、《兽医诊疗新技术》。
四、近五年主持的主要科研项目
1．PEDV下调HNRNPA3影响细胞脂质代谢促进病毒复制复合体形成的机制研究，国家自然基金项目（32373003），2024 -2027，主持人。
2．秦巴山区奶山羊产业及羊乳新产品加工关键技术研究示范，2021年国家重点研发计划（2021YFD1600704）子项目，2022.12-2026.11，子项目主持人.
3．规模化肉牛场疫病防控技术项目，杨凌秦宝牛业有限公司横向课题，2023.9-2026.9，主持人 。
4．奶绵羊围产期疾病防控技术集成研究，2021年甘肃金昌市科技揭榜挂帅制项目（202100-001），2021 -2023，主持人。
5．检测奶山羊多种常见病原的多重实时荧光定量TaqMan PCR检测方法的建立及应用，陕西省重点研发计划项目（2021NY-024），2021-2022，主持人。
6．规模化奶山羊养殖场疫病快速检测技术的集成与示范推广，2020年度西北农林科技大学试验示范站（基地）科技创新与成果转化项目（TGZX2020-12） 2020-2021，主持人。
7．规模化羊养殖场主要疫病防控关键技术集成与示范，陕西省农业厅科技创新驱动项目（NYKJ-2018-YL02），2018-2020，主持人。
8．猪流行性腹泻综合防控关键技术研究与示范，西安市农业科技创新计划(2017050NC/NY010），2017.1-2019.12，主持人。
9．羊常见病原感染可视化LAMP快速检测技术的研究与推广，2017年西北农林科技大学试验示范站(基地)科技成果推广项目，2017.6-2019.5，主持人。
五、代表性学术成果（含论文、专利、标准和著作）
（一）主要研究论文
1．Naling Yang , Qi Zhang, Quanqiong Wang, Yanxia Zhang, Shifan Li, Yina Zhao, Xiaojie Shi, Qinfan Li , Xingang Xu（通讯作者）. Nsp10-interacting host protein SAP18 restricts PEDV replication in Marc-145 cells via enhancing dephosphorylation of RIG-I. Veterinary Microbiology, 2024, 294,110124.
2．Siyuan Li, Jin Huang, Xuhang Cai, Li Mao, Lingling Xie , Fu Wang , Hua Zhou, Xuesong Yuan, Xinru Sun , Xincheng Fu , Baochao Fan, Xingang Xu（通讯作者）, Jizong Li, Bin L. Prevalence and Evolutionary Characteristics of Bovine Coronavirus in China. Vet. Sci. 2024, 11, 230.
3．Xiaojie Shi, Qi Zhang, Naling Yang, Quanqiong Wang, Yanxia Zhang, Xingang Xu(通讯作者）. PEDV inhibits HNRNPA3 expression by miR-218-5p to enhance cellular lipid accumulation and promote viral replication, mBio, 2024, 15(2):e0319723.
4．Linjie Li , Bingqing Li , Jin Wang , Lei Liu , Yi Li b , Shiqi Sun , Shuanghui Yin , Liping Zhang, Xinsheng Liu , Xingang Xu(通讯作者）, HuichenGuo. A novel recombination porcine epidemic diarrhea virus isolated from Gansu, China: Genetic characterization and pathogenicity. Veterinary Microbiology, 2024,290:109975.
5．Yang Liu, Qi Zhang, Min Zou, Jin Cui, Xiaojie Shi, Linjie Li, Faxing Wu, Xingang Xu.（通讯作者）Cell entry of Bovine herpesvirus-1 through clathrin- and caveolin-mediated endocytosis requires activation of PI3K-Akt-NF-κB and Ras-p38 MAPK pathways as well as the interaction of BoHV-1 gD with cellular receptor nectin-1. Veterinary Microbiology, 2023, 279, 109672.
6．Xingang Xu（第一作者）, Yi Liu, Jie Gao, Xiaojie Shi, Yuchao Yan, Naling Yang, Quanqiong Wang, Qi Zhang. GRAMD4 regulates PEDV-induced cell apoptosis inhibiting virus replication via the endoplasmic reticulum stress pathway, Veterinary Microbiology, 2023, 279,109666 .
7．Hongchao Zhou, Yuchao Yan, Jie Gao, Mingrui Ma, Yi Liu, Xiaojie Shi, Qi Zhang, Xingang Xu（通讯作者）. Heterogeneous Nuclear Protein U Degraded the m6A Methylated TRAF3 Transcript by YTHDF2 To Promote Porcine Epidemic Diarrhea Virus Replication. Journal of Virology, 2023, 97(2):e0175122.
8．Xingang Xu（第一作者）, Mingrui Ma , Xiaojie Shi, Yuchao Yan, Yi Liu, Naling Yang, Quanqiong Wang, Shuxia Zhang, Qi Zhang. The novel Nsp9-interacting host factor H2BE promotes PEDV replication by inhibiting endoplasmic reticulum stress-mediated apoptosis. Veterinary Research , 2023, 54(1):27.
9．Xiaojie Shi , Qi Zhang , Jingjing Wang, Yuting Zhang, Yuchao Yan, Yi Liu, Naling Yang, Quanqiong Wang and Xingang Xu（通讯作者）.Diferential expression analysis of mRNAs, lncRNAs, and miRNAs expression profles and construction of ceRNA networks in PEDV infection. BMC Genomics, 2022, 23（1）:586.
10．Zhang Q, Yang F, Gao J, Zhang W, Xu X（通讯作者）. Development of multiplex TaqMan qPCR for simultaneous detection and differentiation of eight common swine viral and bacterial pathogens. Brazilian Journal of Microbiology, 2022,53(1):359–368.
11．Xingang Xu（第一作者）, Lixiang Wang, Yi Liu, Xiaojie Shi, Yuchao Yan, Shuxia Zhang, Qi Zhang. TRIM56 overexpression restricts porcine epidemic diarrhoea virus replication in Marc-145 cells by enhancing TLR3-TRAF3-mediated IFN-β antiviral response. Journal of General Virology, 2022;103(5):001748.
12．Hongchao Zhou, Yuting Zhang, Jingjing Wang, Yuchao Yan, Yi Liu, Xiaojie Shi, Qi Zhang, Xingang Xu（通讯作者）.The CREB and AP-1-Dependent Cell Communication Network Factor 1 Regulates Porcine Epidemic Diarrhea Virus-Induced Cell Apoptosis Inhibiting Virus Replication Through the p53 Pathway. Frontiers in Microbiology, 2022 ,13:831852.
13．Xingang Xu(第一作者), Jingjing Wang, Yuting Zhang, Yuchao Yan, Yi Liu, Xiaojie Shi, Qi Zhang. Inhibition of DDX6 enhances autophagy and alleviates endoplasmic reticulum stress in Vero cells under PEDV infection, Veterinary Microbiology, 2022,266:109350.
14．Mingzhe Fu, Yuchao Yan, Hong Su, Jingjing Wang, Xiaojie Shi, Hongchao Zhou, Qi Zhang , Xingang Xu（通讯作者）. Spleen proteome profiling of dairy goats infected with C. pseudotuberculosis by TMT-based quantitative proteomics approach. Journal of Proteomics, 2021, 248:104352.
15．Pei Sun , Jian Jin, Lixiang Wang , Jingjing Wang , Hongchao Zhou , Qi Zhang , Xingang Xu （通讯作者） .Porcine epidemic diarrhea virus infections induce autophagy in Vero cells via ROS-dependent endoplasmic reticulum stress through PERK and IRE1 pathways. Veterinary Microbiology, 2021 ,253:108959
16．Mingzhe Fu, Hong Su, Zhanqiang Su , Zheng Yin, Jian Jin , Lixiang Wang , Qi Zhang , Xingang Xu （通讯作者）.Transcriptome analysis of Corynebacterium pseudotuberculosis-infected spleen of dairy goats. Microbial Pathogenesis. 2020 (147) 104370.）
17．XingangXu（第一作者）, YingXu, Qi Zhang, FengYang, ZhengYin, Lixiang Wang, QinfanLi. Porcine epidemic diarrhea virus infections induce apoptosis in Vero cells via a reactive oxygen species (ROS)/p53, but not p38 MAPK and SAPK/JNK signalling pathways. Veterinary Microbiology, 2019,232:1-12.
18．Xingang Xu（第一作者）, Feng Yang, Qi Zhang, Ying Xu, Jiali Huang, Mingzhe Fu, Weimin Zhang.Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats. Journal of Viroloical Methods, 2019,266 : 58-64.
19．Pei Sun, Qarih Fahd, Yezhen Li, Yao Sun, Jie Li, Majjid A. Qaria, Zhan Song He, Yuzhen Fan, Qiang Zhang, Qianming Xu, Zongjun Yin, Xingang Xu（通讯作者）, Yu Li. Transcriptomic analysis of small intestinal mucosa from porcine epidemic diarrhea virus infected piglets ,Microbial Pathogenesis, 2019,132:73-79 .
20．Hongqing Zheng , Lei Xu , Yuzhong Liu, Cheng Li , Liang Zhang, Tao Wang , Di Zhao , Xingang Xu（通讯作者）, Yanming Zhang. MicroRNA-221-5p Inhibits Porcine Epidemic Diarrhea Virus Replication by Targeting Genomic Viral RNA and Activating the NF-κB Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES( Int. J. Mol. Sci. ), 2018, 19, 3381.
21．Pei Sun, Haoyang Wu, Jiali Huang, Ying Xu, Feng Yang, Qi Zhang, Xingang Xu（通讯作者）. Porcine epidemic diarrhea virus through p53-dependent pathway causes cell cycle arrest in the G0/G1 phase,Virus Research, 2018 (253) 1-11.
22．Qi Zhang, Ying Xu, Rong Chang, Dewen Tong，Xingang Xu（通讯作者）. Transmissible gastroenteritis virus N protein causes endoplasmic reticulum stress, up-regulates interleukin-8 expression and its subcellular localization in the porcine intestinal epithelial cell. Research in Veterinary Science,2018, 2018 (119) 109-115.
23．Q. ZHANG, J. L. HUANG, Y. B. LIANG,Y. P. HE, D. W TONG, X. G. XU（通讯作者）. Transmissible gastroenteritis virus nsp7 protein localized in down-regulates interleukin 8 expression in porcine intestinal the cytoplasm epithelial cell. Acta virologica .2018 ,62: 41-49.
24．Ya-Peng He，Qi Zhang，Ming-Zhe Fu，Xin-Gang Xu（通讯作者）.Development of multiplex PCR for simultaneous detection and differentiation of six DNA and RNA viruses from clinical samples of sheep and goats. Journal of Virological Methods, 2017,243:44-49.
25．Yapeng He, Qi Zhang, Jing Wang, Man Zhou, Mingzhe Fu, Xingang Xu（通讯作者）. Full-length genome sequence analysis of enzootic nasal tumor virus isolated from goats in China.Virology Journal, 2017,14:141.
26．Xuefeng Qi，Caihong Liu，Ruiqiao Li, Huizhu Zhang, Xingang Xu*（通讯作者），Jingyu Wang. Modulation of the innate immune-related genes expression in H9N2 avian influenza virus-infected chicken macrophage-like cells (HD11)in response to Escherichia coli LPS stimulation, Research in Veterinary Science, 2017 (111) 36-42.
27．Yu-Xin Gong, Bin Zhu, Guang-Lu Liu, Lei Liu, Fei Ling, Gao-Xue Wang, Xin-Gang Xu*(通讯作者). Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effects of a recombinant vaccine against Aeromonas hydrophila. Fish and Shellfish Immunology. 2015, 42（1）: 213-220.
28．Wang J, Tang C, Wang Q, Li R, Chen Z, Han X, Wang J, Xu X*(通讯作者). Apoptosis induction and release of inflammatory cytokines in the oviduct of egg-laying hens experimentally infected with H9N2 avian influenza virus. Vet Microbiol. 2015 ,12;177(3-4):302-314.
29．Xu X（第一作者）, Zhang H, Zhang Q, Rong C, Huang Y, Tong D. Porcine epidemic diarrhea virus M protein blocks cell cycle progression at S-phase and subcellular localization in the porcine intestinal epithelial cell. Acta virologica, 2015,59（3）:265-275.
30．Xu X（第一作者）, Zhang H, Zhang Q, Huang Y, Dong J, Liang Y, Liu HJ, Tong D. Porcine epidemic diarrhea virus N protein prolongs S-phase cell cycle, induces endoplasmic reticulum stress, and up-regulates interleukin-8 expression. Vet Microbiol. 2013,164(3-4):212-21.
31．Xu X（第一作者）, Zhang H, Zhang Q, Dong J, Liang Y, Huang Y, Liu HJ, Tong D. Porcine epidemic diarrhea virus E protein causes endoplasmic reticulum stress and up-regulates interleukin-8 expression. Virol J. 2013,19;10:26.
32．Xin-Gang Xu（第一作者）, Zhi-Sheng Wang, Qi Zhang, Zhao-Cai Li, Li Ding, Wei Li, Hung-Yi Wu, Ching-Dong Chang, Long-Huw Lee, De-Wen Tong, Hung-Jen Liu . Baculovirus as a PRRSV and PCV2 bivalent vaccine vector: Baculovirus virions displaying simultaneously GP5 glycoprotein of PRRSV and capsid protein of PCV2 , Journal of Virological Methods, 2012,179(2), 359-366
33．Xin-Gang Xu（第一作者）, Hong-Ni Zhao, Qi Zhang, Li Ding, Zhao-Cai Li, Wei Li, De-Wen Tong*, Hung-Jen Liu*. Oral vaccination with attenuated Salmonella enterica serovar Typhimurium expressing Cap protein of PCV2 and its immunogenicity in mouse and swine models. Veterinary Microbiology. 2012, 157(3-4):294-303.
34．Xin-Gang Xu（第一作者）, Guang-Da Chen , Yong Huang, Li Ding , Zhao-Cai Li, Ching-Dong Chang, De-Wen Tong* , Hung-Jen Liu*. Development of multiplex PCR for simultaneous detection of six swine DNA and RNA viruses. Journal of Virological Methods, 2012,183(1):69-74.
35．Xu X（第一作者）, Zhang K, Huang Y, Ding L, Chen G, Zhang H, Tong D. Bovine herpes virus type 1 induces apoptosis through Fas-dependent and mitochondria-controlled manner in Madin-Darby bovine kidney cells. Virology Journal. 2012 ,17;9:202.
36．Zhao-Cai Li, Xin-Gang Xu (并列第一), Yong Huang , Li Ding, Zhi-Sheng Wang ,Gao-Shui Yu, Dan Xu, Wei Li, Dewen Tong*. Swainsonine activates mitochondria-mediated apoptotic pathway in human lung cancer A549 cells and retards the growth of lung cancer xenografts. International Journal of Biological Sciences, 2012; 8(3):394-405.
37．Li Ding, Xin-Gang Xu (并列第一), Yong Huang , Zhao-Cai Li, Zhi-Sheng Wang ,Gao-Shui Yu, Dan Xu, Wei Li, Dewen Tong*. Transmissible gastroenteritis virus induces apoptosis through FasL and mitochondrion-mediated pathway dependent of viral replication. Veterinary Microbiology. 2012, 158(1-2):12-22.
38．Xin-Gang Xu（第一作者）, Zhi-Sheng Wang, Qi Zhang, Zhao-Cai Li, Hong-Ni Zhao, Wei Li, De-Wen Tong, Hung-Jen Liu. Baculovirus surface display of E envelope glycoprotein of Japanese encephalitis virus and its immunogenicity of the displayed proteins in mouse and swine models, Vaccine, 2011, 29(4):636-643.
39．Xin-Gang Xu（第一作者）, Zhi-Sheng Wang, Qi Zhang , Zhao-Cai Li, Kuan Zhang, Wei Li, De-Wen Tong , Hung-Jen Liu. Baculovirus virions displaying infectious bursal disease virus VP2 protein protect chickens against infectious bursal disease virus infection. Avian Diseases, 2011，55:223–229
40．Zhi-sheng Wang, Xin-gang Xu（并列第一）, Hung-jen Liu, Zhao-cai Li1, Li Ding, Gao-shui Yu1, Dan Xu and Dewen Tong. Immunogenicity of envelope GP5 protein displayed on baculovirus and protective efficacy against virulent porcine reproductive and respiratory syndrome virus challenge in piglets, African Journal of Microbiology Research, 2011, 5(18): 2682-2691.
41．ZS WANG, XG XU(并列第一), H.J LIU, ZC LI, L. DING, G.S YU, DW TONG1. Immunogenicity of envelope GP3 protein of porcine reproductive and respiratory syndrome virus displayed on baculovirus， Acta virologica . 2011，55:139 -146.
42．Xin-Gang Xu（第一作者）, De-Wen Tong, Ming-Tang Chiou, Yao-Ching Hsieh, Wen-Ling Shih, Ching-Dong Chang, Ming-Huei Liao, Yan-Ming Zhang, Hung-Jen Liu. Baculovirus surface display of NS3 nonstructural protein of classical swine fever virus. Journal of Virological Methods, 2009, (159)2: 259-264.
43．Xin-Gang Xu（第一作者）, Ming-Tang Chiou, Yan-Ming Zhang, De-Wen Tong, Jian-He Hu, Miao-Tao Zhang, Hung-Jen Liu. Baculovirus surface display of Erns envelope glycoprotein of classical swine fever virus. Journal of Virological Methods, 2008, 153(2): 149-155.
44．Xin-Gang Xu（第一作者）, Hung-Jen Liu. Baculovirus surface display of E2 envelope glycoprotein of classical swine fever virus and immunogenicity of the displayed proteins in a mouse model. Vaccine, 2008, 26(43): 5455-5460.
（二）申报及获批国家发明专利
1．表达PRRSV免疫原基因的重组杆状病毒及其制备和应用，发明专利， 专利号：ZL201010140792.4；专利授权日期: 2012.5.30。
2．表达PCV-2免疫原基因的重组杆状病毒及其制备和应用，发明专利，专利号：ZL201010140804.3；专利授权日期: 2012.7.18。
3．一种用于同时检测绵羊和山羊六种病毒的多重PCR试剂盒,发明专利，专利号： 201510810268.6 ；专利授权日期: 2019.4.28。
4．一种快速检测奶牛子宫内膜炎中病原菌的方法，发明专利，专利号：ZL 201910186379.2；专利授权日期: 2022.5.。
（三）主要论著
1．《兽医微生物实验实习指导》，西北农林科技大学出版社， 2020.12，主编，ISSN978-7-5683-0887-8。
2．《常用实验动物传染病》，甘肃科学技术出版社，2020.12，许信刚副主编，ISN978-7-5424-2758-8。
3．奶山羊实用养殖技术之奶山羊常见疫病防治技术，西北农林科技大学出版社，2021.9，本册主编许信刚，ISSN978-7-5683-0961-5。
4．《兽医微生物学》第二版，高等教育出版社，2023.7，参编， ISBN 978-7-04-060807-6。
5．《奶绵羊应用生产学》，中国农业出版社，2022.11, 参编，ISBN 978-7-109-30134-4。
6．奶山羊饲养管理关键技术，西北农林科技大学出版社，2023,7，参编，ISBN 978-7-5683-1279-0
五、荣誉及奖项
1．“鸡传染性法氏囊病综合防治技术研究”获2000年度陕西省农业厅“陕西省农牧业科学技术进步一等奖”；
2．“鸡传染性法氏囊病综合防治技术研究”获2001年度陕西省人民政府“陕西省科学技术进步一等奖”；
3．2006动物科技学院青年教师讲课比赛一等奖；
4．2008动物医学院年学术报告优秀论文二等奖；
5．2009度年动物医学院优秀教师；
6．2009年动物医学院青年教师讲课比赛二等奖；
7．2009年动物医学院学术报告优秀学术报告一等奖；
8．2016年第4届全国大学生动物医学专业技能大赛（生泰尔杯）特等奖，指导教师。
9．2018年第5届全国大学生动物医学专业技能大赛（生泰尔杯）特等奖，指导教师。
10．2020年第6届全国大学生动物医学专业技能大赛（生泰尔杯）特等奖，指导教师。
11．2020年11月，被安康市科学技术局评为安康市“三区”科技人才工作先进个人。
12．2019年10月，被安康市科学技术局评为安康市“三区”科技人才工作先进个人。
13．2022年4月，“奶山羊健康养殖技术研究集成及推广”获陕西农业技术推广成果奖二等奖（排序第8）
六、联系方式
通信地址：陕西.杨凌.西北农林科技大学；712100；
邮箱：286567031@qq.com